miRNA-429 regulates the proliferation and migration of human breast cancer cell line Bcap37

doi:10.3877/cma. j. issn.1674-0807.2015.02.004

Abstract

Abstract:

Objective

To investigate the effect of miRNA-429 on proliferation and migration of human breast cancer Bcap37 cells after overexpressing miRNA-429.

Methods

The miRNA-429-overexpressed lentivirus and the blank lentivirus were infected into human breast cancer Bcap37 cells to establish stably transfected cells as the experimental group and the control group respectively. Real-time PCR was used to detect the expression level of miRNA-429 after infection;MTT assay was used to detect the effect of miRNA-429 on cell proliferation of Bcap37; the scratch test and Transwell experiment were used to detect the effect of miRNA-429 on migration ability of Bcap37 cells. The data from over 2 groups were analyzed by one-way variance analysis and pairwise comparison was conducted by LSD-t test. The data between two groups were compared by t test. The data from multiple time points were compared by analysis of variance of repeated measurement.

Results

After infecting the miRNA-429-overexpressed lentivirus, the expression level of miRNA-429 in Bcap37 cells of experimental group was significantly up-regulated compared with the control group(2.88±0.02 vs 1.00 ±0.05,t=10.33,P=0.009). The proliferation ability of Bcap37 cells in experimental group was enhanced compared with the control group (F=189.410,P=0.000). The scratch test and Transwell experiment showed that the migration ability of Bcap37 cells was increased after overexpression of miRNA-429 compared with the control group(605±23 vs 365±21,t=13.30,P=0.001).

Conclusion

The overexpressed miRNA-429 can significantly increase the proliferation and migration abilities in human breast cancer cell line Bcap37.

Key words: Breast carcinoma, MicroRNAs, Cell proliferation, Cell movement

Keyu Qin, Hengyu Li, Lu Xu, Yuan Sheng. miRNA-429 regulates the proliferation and migration of human breast cancer cell line Bcap37[J]. Chinese Journal of Breast Disease(Electronic Edition), 2015, 09(02): 89-95.

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URL: https://zhrxbzz.cma-cmc.com.cn/EN/10.3877/cma. j. issn.1674-0807.2015.02.004

https://zhrxbzz.cma-cmc.com.cn/EN/Y2015/V09/I02/89

Figures/Tables 5

References 25

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细胞	实验次数	miRNA-429相对表达量
Bcap37	3	1.010±0.006^a
BT474	3	1.197±0.032
SK-BR-3	3	1.243±0.020
MDA-MB-231	3	1.197±0.094
MCF-7	3	1.230±0.060
BT20	3	3.503±0.009
T47D	3	5.057±0.009
F值		1675.114
P值		0.000

细胞	实验次数	miRNA-429相对表达量
Bcap37	3	1.010±0.006^a
BT474	3	1.197±0.032
SK-BR-3	3	1.243±0.020
MDA-MB-231	3	1.197±0.094
MCF-7	3	1.230±0.060
BT20	3	3.503±0.009
T47D	3	5.057±0.009
F值		1675.114
P值		0.000

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